Comparative evaluation of coronal discoloration induced by two triple antibiotic revascularization protocols when used at varying depths of temporary sealing material at the end of varying time periods.

AIM: The aim of this study is to evaluate and compare the coronal discoloration induced by two Triple antibiotic paste (TAP 1 and 2), when used with varying depths of 2 and 4 mm of temporary sealing materials at the end of 0, 3, and 6 weeks. OBJECTIVES: (1) To evaluate coronal discoloration induced by TAP-1 when the depth of the temporary restorative material is 2 mm below cementoenamel junction at the end of 0, 3, and 6 weeks. MATERIALS AND METHODS: Forty extracted permanent maxillary central and lateral incisors or single-rooted tooth were collected and chosen for the study. They were then divided into two groups and each group was then subdivided into two subgroups. RESULTS: Both the triple antibiotic paste showed discoloration but it reduced with increase in thickness of temporary cement. CONCLUSION: TAP 1 and TAP 2 both showed an increase in discoloration, greater discoloration was seen with TAP, containing minocycline. There was a steady change in the discoloration from 0 to 6th week. In both the groups, TAP 1 and TAP 2, there was a reduction in discoloration with increase thickness of the temporary restorative material.

Comparative Evaluation of Cytotoxicity of Root Canal Sealers on Cultured Human Periodontal Fibroblasts: In vitro Study.

AIM: To evaluate and compare the cytotoxic effects of different types of root canal. MATERIALS AND METHODS: The sealers were eluted with culture medium for 1 hour, 7 days, and 14 days. Cell viability was estimated by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and trypan blue exclusion method on human periodontal ligament (PDL) fibroblast cells. Sealers used are mineral trioxide aggregate (MTA)-based sealer (MTA Fillapex, Angelus), calcium hydroxide-based sealer (Apexit Plus, Ivoclar Vivadent), resin-based sealer (AH Plus, Dentsply), and zinc oxide eugenol-based sealer (Tubli Seal, SybronEndo). RESULTS: The order of cytotoxicity through MTT assay, at the end of the second week, was observed as MTA Fillapex> Tubli Seal> Apexit Plus > AH Plus. The percentage cell viability obtained after trypan blue exclusion method decreased in the order of Apexit Plus> Tubli Seal> AH Plus> MTA Fillapex, which was similar to the reported cytotoxicity from the MTT assay after 1 hour. CONCLUSION: Each type of sealer showed moderate-to-severe cytotoxic response when compared with the control. The MTA Fillapex was found to be the most cytotoxic sealer. Use of resin-based material as a root canal sealer may result in a more favorable response to PDL fibroblasts. CLINICAL SIGNIFICANCE: Having knowledge of the cytotoxicity of various sealers will help in increasing patient's comfort.